Fabrication of conductive Ag/AgCl/Ag nanorods ink on Laser-induced graphene electrodes on flexible substrates for non-enzymatic glucose detection

Abstract

An unusual strategy was designed to fabricate conductive patterns for flexible surfaces, which were utilized for non-enzymatic amperometric glucose sensors. The Ag/AgCl/Ag quasi-reference ink formulation utilized two reducing agents, NaBH_{4} and ethylene glycol. The parameters of the ink, such as sintering time and temperature, NaBH_{4} concentration, and layer number of coatings on flexible laser-induced graphene (LIG) electrodes were investigated. The conductive Ag/AgCl/Ag ink was characterized using electrochemical and surface analysis techniques. The electrocatalytic activity of Ag/AgCl/Ag NRs can be attributed to their high surface area, which offer numerous active sites for catalytic reactions. The selectivity and sensitivity of the electrodes for glucose detection were investigated. The XRD analysis showed (200) oriented AgCl on covered Ag NRs, and with the addition of NaBH_{4}, the intensity of the peaks of the Ag NRs increased. The wide linear range of non-enzymatic sensors was attained from 0.003 to 0.18 mM and 0.37 to 5.0 mM, with a low limit of detection of 10 upmuM and 20 upmuM, respectively.The linear range of enzymatic sensor in real sample was determined from 0.040 to 0.097 mM with a detection limit of 50 upmuM. Furthermore, results of the interference studies demonstrated excellent selectivity of the Ag/AgCl/Ag NRs/LIG electrode. The Ag/AgCl/Ag NRs on the flexible LIG electrode exhibited excellent sensitivity,long-time stablity,and reproducibility. The efficient electroactivity were deemed suitable for various electrochemical applications and biosensors.