Fabrication of Chitosan-Nano Hydroxyapatite Scaffold for Dental Tissue Engineering

Abstract

Incorporation of hydroxyapatite (HA) with organic polymer would be used in bone and dental tissue engineering. According to prior researches, because of its chemical similarity to natural bone and dental, this scaffold could improve bioactivity and bone bonding ability. In this research, nano-hydroxyapatite/ chitosan scaffolds were synthesized by two different methods to investigate the effect of different method on scaffold properties. Morphological properties of scaffold such as pore size and fibers diameter were investigated using SEM evaluation, and also bioactivity of scaffolds were measured in simulated body fluid (SBF). The results indicate that Chitosan/nano-Hydroxyapatite scaffold might be a suitable scaffold for tooth engineering while electrospinning techniques present more flexibility and controllable pore size than freeze-drying while with freeze-drying technique percentage of usage hydroxyapatite could be risen up to 40% and shown better macro-mechanical and physical properties.