Abstract
This present paper describes a new approach to fabricate a new amperometric sensor for the determination of glucose. Polyaniline (PANI) film doped with colloidal gold nanorod particles has been used to immobilize glucose oxidase by glutaraldehyde. The polyaniline/gold nanorod composite structure gave an excellent matrix for enzyme immobilization due to the large specific surface area and higher electroactivity. The composite has been characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM), and surface-enhanced Raman spectroscopy (SERS). The SERS spectrum of the surface-immobilized glucose oxidase and the spectrum of the native enzyme indicate that the main feature of the native structure of glucose oxidase was conserved after being immobilized on the polymer matrix. The amperometric response was measured as a function of concentration of glucose at a potential of 0.6 V versus Ag/AgCl in 0.1 M phosphate buffer at pH 6.4. Linear range of the calibration curve was from 17.6 μM to 1 mM with a sensitivity of 13.8 μA⋅mM−1⋅cm−2and a limit of detection (LOD) of 5.8 μM. The apparent Michaelis-Menten constantKMwas calculated as 1.0 mM and the response time was less than 3 seconds.
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