Fabrication of biohybrid electrospun nanofibers for the eradication of wound infection and drug-resistant pathogens

Abstract

There is a current need for the development of novel wound dressings with antibacterial activity for the treatment of pathogenic bacteria. The present study aimed to fabricate biohybrid electrospun nanofibers via encapsulation of novel bioactive molecules (BM) extracted from marine Streptomyces sp. (mS) into poly(vinyl alcohol) (PVA) nanofibers using electrospinning techniques. The morphology and chemical characteristics of the fabricated mS/PVA nanofibers that incorporated with various ratios of BM extract were studied using various techniques. Determination of the chemical composition of BM extract using gas chromatography-mass spectrometry (GC–MS) revealed that it was composed of 1H-pyrrole-2-carboxylic acid (31.83 %), phenylmalonic acid (21 %), and pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) (12.82 %). Interestingly, mS/PVA with a smooth and cylindrical structure, exhibited potent antibacterial activity against the bacteria; S. aureus, E. coli, and drug-resistant S. epidermidis at a concentration of 5% w/w of the extract. Hence, biohybrid mS/PVA nanofibers are a potential material that can be applied for complete eradication of wound infection and drug-resistant pathogens. Notably, biohybrid mS/PVA nanofibers could be a promising alternative antimicrobial agent for metal nanoparticles in medical applications, especially for the wound dressing purposes.