Fabrication of asymmetric nanostarch reinforced Chitosan/PVP membrane and its evaluation as an antibacterial patch for in vivo wound healing application

Abstract

Starch is an abundant, relatively inexpensive and ecofriendly materials which can be easily convert into nanoparticle and also as filler for the preparation of bionanocomposite for wound dressing application. Symmetric and asymmetric Chitosan(C)/PVP(P) films containing porous structure supported with nanostarch (NS) were prepared by salt leaching method for wound dressing application. Symmetric Chitosan/PVP/Nanostarch (CPNS) film with 1% and 3% wt nanostarch was prepared without coating of stearic acid whereas asymmetric Chitosan/PVP/Nanostarch-Stearic acid (CPNS-S) film was prepared by coating of stearic acid. The stearic acid coated surface possesses hydrophobic water repellent, microporous, bacterial anti adhesion property and the stearic acid uncoated hydrophilic surface shows superior antibacterial and noncytotoxicity property with highly porous character. All the symmetric and asymmetric films exhibit almost same mechanical, barrier, swelling and hemolytic property reveals that the stearic acid does not affect the physical and hemolytic property whereas the concentration of nanostarch greatly influence the above property. The reinforcement of nanostarch with chitosan and PVP was proved by TEM and SEM analysis. The CPNS1%-S film shows excellent S. aureus anti adhesion property. Furthermore, the in vivo excision-type wound healing proved that the CPNS1%-S film enhanced the healing effect and increased re-epithelialization and collagen formation.