Fabrication of acid-resistant imprinted layer on magnetic nanomaterials for selective extraction of chlorogenic acid in Honeysuckle

Abstract

The common elution process of molecularly imprinted polymers (MIPs) is carried out in an acidic medium, which greatly affects the stability and reusability of synthetic MIPs, especially for magnetic MIPs. In this study, we fabricated an acid-resistant imprinted layer formed by phase-transitioned lysozyme on magnetic nanomaterials for selective extraction of chlorogenic acid in Honeysuckle, which often coexists with structural analogs. The newly designed acid-resistant imprinted layer can not only protect the internal magnetic core from denudation and dissolution, but also maintain the integrity of the imprinted layer during the elution process. The resultant magnetic MIPs exhibited good stability with no change on morphology after the repeatedly eluting process, and satisfactory reusability that can be used at least ten adsorption-desorption cycles with almost no decrease for adsorption capacity. In addition, the resultant materials possess satisfactory magnetism, uniform morphology with typical core-shell structure, stable crystallization, and good adsorption performance showing on high adsorption amount (10.82 mg g−1), fast kinetic equilibrium time (as short as 30 min), and satisfactory selectivity (IF = 2.85, SC > 1.5). At last, the obtained magnetic MIPs as adsorbents coupled with HPLC were successfully used to selective extract CGA in Honeysuckle samples with the high recoveries in the range of 92.0–104.4%, and the contents of CGA in Honeysuckle samples from the different origin are calculated in the range of 0.98%–1.24%.