Abstract
Accurate diagnosis of breast cancer cells is important on determining the stage of the disease and designing the suitable therapies. Herein, we report a sensitive biosensor based on surface plasmon resonance (SPR) spectroscopy for the detection of breast cancer cells (MCF-7). Firstly, calix[4]arene crownether was used to form a self-assembled monolayer (SAM) on the gold surface. The designed peptide containing recognization and spacer parts was immobilized on the calixarene monolayer surface through host-guest interactions between the ionized amine group of lysine and crown moiety orientedly. The peptide can recognize and capture target breast cancer cells on the surface selectively and sensitively. The detection limit can be achieved as low as 197 cells mL−1. The phenomenon in experiments can be characterized through the intuitional SPR signal increase by the SPR instrument. The fabricated simple and convenient MCF-7 sensor is proposed for application in clinical analysis.
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