Abstract
How to detect uric acid is an important issue. For the purpose of preparing a potentiometric uric acid biosensor, this research used nickel oxide (NiO) as the sensing film to deposit it onto the substrate by radio frequency sputtering, then modified it with reduced graphene oxide (rGO) and silver (Ag) nanowires. Reduced graphene oxide (rGO) not only has excellent electrical conductivity, but also can make the surface of the film have a larger surface area, while AgNWs have also been proven to improve catalytic activity; hence, these two materials were chosen as sensor modifiers. Finally, the stability and the various characteristics of the uric acid biosensor were investigated using a voltage–time (V–T) system. The results showed that the AgNW–uricase/rGO/NiO uric acid biosensor has average sensitivity with 4.66 mV/(mg/L). In addition, the sensor has good stability.
Highlights
Biosensors are an analysis system or device composed of biosensing components, including an enzyme [1] and an antibody [2]
In order to improve the performance of the sensor, we propose a potentiometric enzymatic biosensor that relies on physical adsorption to immobilize an enzyme on the nickel oxide (NiO) sensor in order to measure uric acid concentrations in a solution, and use modifiers to enhance the performance of the sensor
roughness average (Ra) represents the roughness of surfaces, while Rq describes the standard deviation of the contour heights, and permits the surface roughness to be confirmed by statistical methods [35]
Summary
Biosensors are an analysis system or device composed of biosensing components, including an enzyme [1] and an antibody [2]. This study proposes a potentiometric biosensor that relies on physical adsorption to immobilize an enzyme on the NiO-based sensor in order to measure uric acid concentrations in a solution. If human body is in an abnormal state—such as abnormal metabolism, gout, high blood sugar, or other symptoms—it may cause the body’s uric acid concentration to reach a high level. This result causes irreversible damage to the human body. In order to improve the performance of the sensor, we propose a potentiometric enzymatic biosensor that relies on physical adsorption to immobilize an enzyme on the NiO sensor in order to measure uric acid concentrations in a solution, and use modifiers to enhance the performance of the sensor
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