Fabrication of a cycloalkyl-monolith for on-line solid-phase extraction and determination of four polyphyllins in plasma

Abstract

A cycloalkyl-based polymer monolithic column for solid-phase extraction was prepared via radical polymerization using cyclohexyl methacrylate as the monomer. The preparative conditions such as crosslinker/monomer ratio and the amount of the porogens were optimized and the resulting monoliths were characterized by scanning electron microscopy and nitrogen adsorption-desorption method. On-line solid-phase extraction-high-performance liquid chromatography was performed to quantitatively analyse polyphyllin I, II, VI and VII contained in herbal medicine of paridis rhizome in mouse plasma using the homemade optimized monolithic SPE column combined with a C18 column, in which water was used to remove the plasma matrix while the polyphyllins in the mouse plasma were eluted by acetonitrile-water (42:58, V/V). Results obtained from the method validation show that the present method is feasible for the quantitative analysis of the four polyphyllins in plasma. The developed method was further applied for the real mouse plasma sample. These results show that the homemade cycloalkyl-based polymer monolithic SPE column has good ability for clean-up of the interfering bio-matrix and simultaneously extracting the four polyphyllins from mouse plasma. Furthermore, the present method is a promising method for quantitative determination of saponins compounds from complex bio-samples with the advantages of simple and efficient.