Fabrication, Evaluation and In-vitro, In-vivo Efficacy of Electrospun Nanofiber Mats of Tinospora cordifolia Extract and Curcumin

Abstract

The current research work highlights the fabrication and evaluation of nanofiber mats prepared using electrospinning technology containing Tinospora cordifolia extract and curcumin incorporated in chitosan polymer. The study of the prepared mats was carried out to optimize the formulations, to check in-vitro antibacterial activity, and to establish the effectiveness in diabetic wound healing by conducting an animal study. The nanofiber mats of curcumin and leaf extract of T. cordifolia using chitosan as a base polymer were prepared, optimized, and evaluated for antibacterial activity in-vitro, while their ability to enhance the wound healing process was checked using an animal model. The research parameters included the rate at which the wound contracted besides the time taken for epithelialization by using the wound healing model- excision technique. The results we found are encouraging for further studies. The nanofibers showed good antibacterial potential and faster healing of wounds. The research finished by developing an improved new formulation that effectively combats bacterial resistance, a common problem in the diabetic wound healing process. The mixture will not only fight resistant bacteria, but it will also help diabetic wounds heal faster because the T. cordifolia leaf extract is known to improve blood vessel growth and tissue remodeling.