Abstract
ABSTRACT The aim of this study was to synthesize chitosan nanoparticles loaded with rapeseed extract (RPE-CN) and to evaluate its therapeutic effects. The RPE-CNwas synthesized (Ion gelation), characterized (DLS, FTIR, atomic and electron microscopy) and their toxicity was evaluated by MTT method.Anti-inflammatory and pro-apoptotic effects were evaluated by qPCR method. Its antioxidant power was measured by ABTS, DPPH and FRAP methods and CAM method was used to evaluate its anti-angiogenic effects.Treatment with RPE-CN(Ps: 93.81 nm, PDI: 0.3, and ζ p: +35mv) reduced MCF7 cell viability and increased expression of genes associated with apoptosis (Caspase-3 and Caspase-9), inflammation (IL10) and antioxidant (SOD).RPE-CN exhibited notable inhibition in ABTS, DPPH, and Fe3+-TPTZ free radicals. Finally, angiogenesis in CAM tissue was suppressed by the down-regulation of VEGFR gene expression. According to the results, RPE-CN can be introduced as a potential chemo-preventive agent in the treatment of cancer.
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