Fabrication and Release Kinetics of Liposomes Containing Leuprolide Acetate

Sudipta Das,Koushik Bankura,Debatri Roy,Amit Nayak,Arnab Samanta

doi:10.51152/jbarbiomed.v7i1.213

Abstract

The present work is focused on the preparation and in vitro release kinetics of liposomal formulation of Leuprolide Acetate. In this work, “Thin Lipid Film Hydration Method” was used for preparation of Leuprolide Acetate loaded liposomes. Prepared liposomal formulations of Leuprolide acetate was evaluated by drug entrapment study, in-vitro drug release kinetics and stability studies. The percentage drug entrapment of Leuprolide acetate for F1 and F2 formulations were found to be 78.14 ± 0.67 and 66.70 ± 0.81% respectively. In-vitro drug release study of liposomal formulations had shown zero order release pattern. Regression co-efficient (R2) value of Zero order kinetics for F1 and F2 formulations were 0.9912 and 0.9676 respectively. After storing formulations for 1 month, stability testing was done at 40C.It was found that all batches were stable. These liposomal formulations of Leuprolide acetate can be formulated for parenteral application to treat prostate cancer and in women, to treat symptoms of endometriosis (overgrowth of uterine lining outside of the uterus) or uterine fibroids.

Highlights

Liposomes are nowadays very effective and useful delivery system for transmission of drug, enzymes, vaccine etc, in human or animal body (Senthilkumar et al, 2021; Bhattacharjee et al, 2019; Budai et al, 2007)
This result might be attributed to the fact that the viscosity increased with increasing ratio of lecithin to cholesterol used in the preparation
In this study, liposomal formulations of Leuprolide Acetate were prepared by thin lipid film hydration method by using lecithin (L-α-Phosphatidylcholine) and cholesterol

Summary

Introduction

Liposomes are nowadays very effective and useful delivery system for transmission of drug, enzymes, vaccine etc, in human or animal body (Senthilkumar et al, 2021; Bhattacharjee et al, 2019; Budai et al, 2007). Liposomes are eliminated by simple degradation without creating any toxic effect and provide significant advances as compared to other Nano Drug Delivery Systems (Moghimipour et al, 2015; Rathod et al, 2010). Both kinds of drugs like hydrophilic and lipophilic can be entrapped within liposomes for treating different ranges of diseases (Bozzutoet et al, 2015; Xing et al, 2016; Lee et al, 2017; Pal et al, 2010). According to study in the year 2018, 1.3 Million cases of prostate cancer reports were detected from which 3, 59,000 deaths were observed (Das et al, 2020). This study is focused on formulation and evaluation of liposomal formulation of Leuprolide Acetate for parenteral administration

Methods

Results

Conclusion