Abstract
Tissue engineered scaffold was regarded as a promising approach instead of the autograft. In this study, small diameter electrospun collagen tubular scaffold with random continuous smooth nanofibers was successfully fabricated. However, the dissolution of collagen in concentrated aqueous (conc. aq.) acetic acid caused to the serious denaturation of collagen. A novel method ammonia treatment here was adopted which recovered the collagen triple helix structure according to the analysis of IR spectra. Further dehydrothermal (DHT) and glutaraldehyde (GTA) treatments were applied to introduce the crosslinks to improve the properties of collagen tube. The nanofibrous structure of collagen tube in a wet state was preserved by the crosslinking treatments. Swelling ratio and weight loss decreased by at least two times compared to those of the untreated collagen tube. Moreover, tensile strength was significantly enhanced by DHT treatment (about 0.0076 cN/dTex) and by GTA treatment (about 0.075 cN/dTex). In addition, the surface of crosslinked collagen tube kept the hydrophilic property. These results suggest that DHT and GTA treatments can be utilized to improve the properties of electrospun collagen tube which could become a suitable candidate for tissue engineered scaffold.
Highlights
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Zeugolis et al reported that 99% of collagen triple helix structure lost after electrospinning [31]
Theviscoelastic viscoelasticresponse response solution critical for the smooth electrospun fiNative collagen solution behaves as gel because of the intermolecular association
Summary
Nervous and vascular diseases are the prevalent diseases worldwide which cause the functional disability of the patients and death [1,2]. In these cases, the clinical gold standard for the treatment of the injured nerves and diseased vessels is the autograft, which can give its ready availability, microenvironment and native growth factors [3,4]. There can often be associated with sacrificing function at the donor site or lack of suitable donor tissue For these reasons, tissue engineered tubular scaffold, which offsets the inherent disadvantage, has been proposed to be a promising approach to autograft repairs, in short defects [5,6]
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