Fabrication and Evaluation of Lidocaine Hydrochloride loaded Cubosomes

Abstract

Topical delivery of local anaesthetic drugs such as Lidocaine HCl using carriers and novel nanotechnology can enhance effective drug permeation through the skin into deeper layers and exhibit desirable duration of action. The present study was aimed to formulate and evaluate Lidocaine HCl loaded cubosomes (LHLCs) for sustained therapeutic topical action. Cubosomes emanated as favourable means for the delivery of the drug. LHLCs were prepared by top-down technique using lipid and polymer. Eight formulations of LHLCs were prepared using different concentrations of glyceryl monooleate (GMO) and Poloxamer 407 (P-407). Local anaesthetics create loss of sensation in particular region of the body by inhibiting impulse generation and propagation. Lidocaine HCl is most commonly used amino amide local anaesthetic. It is used as local, topical, intravenous, epidural, peripheral and spinal anaesthesia. The prepared cubosomal dispersions were evaluated to determine surface morphology, particle size, poly dispersibility index (PDI), zeta potential, entrapment ability, tissue distribution studies, and in vitro drug release studies. Scanning Electron Microscopic analysis confirmed that drug was encapsulated in bicontinuous structure. The maximum entrapment efficiency was found to be 89.85±1.1% with vesicle size as 228±2.1nm, charge as -5.68±2.7, PDI as 0.295 and 98.83%± 0.12 in vitro drug release at the end of 12 hr for F7 formulation, which was confirmed as optimized cubosomal dispersion.