Fabrication and Evaluation of Carboxy Methyl Cellulose Anchored Dextran Bioinspired Hydrogel for Effective Delivery of Piroxicam

Abstract

The main focus of the work was to develop a hydrogel of carboxy methyl cellulose tailored dextran containing piroxicam intended for transdermal delivery. Firstly, hydrogel was formulated by conjugation of carboxy methyl cellulose with amino dextran and then piroxicam was mixed to encapsulation in the hydrogel matrix. The hydrogel characterization was performed in terms of zeta potential, entrapment efficiency, morphology and in vitro drug release. The size of the particles was found 134 nm of carboxy methyl cellulose-dextran hydrogels and entrapment efficiency was observed as 87.36 %±1.23 % and 72.35 %±2.35 % in case of carboxy methyl cellulose-dextran hydrogels and carboxy methyl cellulose hydrogels, respectively. An in vivo study for piroxicam loaded carboxy methyl cellulose-dextran hydrogels formulation was performed according to more (6.22 mg/ml) than carboxy methyl cellulose hydrogels (plain) formulation concentration in plasma (3.21 mg/ml). Carboxy methyl cellulose hydrogels in case of epidermis, the piroxicam retained was 15.61±1.5 mg/ml and in dermis it was 6.24 %±1.2 % as well as in case of carboxy methyl cellulose-dextran hydrogels in epidermis the piroxicam retention was 5.21±1.19 mg/ml and in dermis was 26.34±0.5 mg/ml in 12 h. Piroxicam loaded hydrogel were found effective in wound healing through improved collagen and protein content, and restoration of antioxidants in granuloma tissues.