Abstract
In the current paper, we fabricated, characterized, and applied nanocomposite hydrogel based on alginate (Alg) and nano-hydroxyapatite (nHA) loaded with phenolic purified extracts from the aerial part of Linum usitatissimum (LOH) as the bone tissue engineering scaffold. nHA was synthesized based on the wet chemical technique/precipitation reaction and incorporated into Alg hydrogel as the filler via physical cross-linking. The characterizations (SEM, DLS, and Zeta potential) revealed that the synthesized nHA possess a plate-like shape with nanometric dimensions. The fabricated nanocomposite has a porous architecture with interconnected pores. The average pore size was in the range of 100–200 µm and the porosity range of 80–90%. The LOH release measurement showed that about 90% of the loaded drug was released within 12 h followed by a sustained release over 48 h. The in vitro assessments showed that the nanocomposite possesses significant antioxidant activity promoting bone regeneration. The hemolysis induction measurement showed that the nanocomposites were hemocompatible with negligible hemolysis induction. The cell viability/proliferation confirmed the biocompatibility of the nanocomposites, which induced proliferative effects in a dose-dependent manner. This study revealed the fabricated nanocomposites are bioactive and osteoactive applicable for bone tissue engineering applications.
Highlights
IntroductionBone fracture is a common condition that everyone may encounter in their life
This article is an open access articleBone fracture is a common condition that everyone may encounter in their life
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Summary
Bone fracture is a common condition that everyone may encounter in their life. The bone tissue can regenerate by itself, but in large defects, it is required to be treated with proper interventions to improve and help the healing process [1]. Autograft is the gold standard clinical treatment for large bone defects, and despite its acceptable treatment outcomes, suffers from critical shortcomings related to the harvesting process. For distributed under the terms and conditions of the Creative Commons. Novel treatment strategies have been trying to propose innovative concepts to eliminate the limitations of the current treatment modalities. Nanotechnology in combination with tissue engineering has evolved as an innovative concept to bypass the need for autograft and propose sophisticated structures as bone healing materials [5,6]
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