Fabrication and characterization of Chrysin – A plant polyphenol loaded alginate -chitosan composite for wound healing application

Abstract

The present study explores the therapeutic efficacy of sodium alginate-chitosan scaffolds loaded with Chrysin (ALG-CS-CHY) for dermal wound management. Scaffolds were prepared by the vacuum freeze-drying method. The physiochemical characterization was done through Fourier Transform Infra-Red Spectroscopy (FTIR), which revealed the interactions between the scaffold's functional groups and the drug. Surface Electron microscopy (SEM) showed a porous architecture varying from 200-400 μm. X-ray Diffraction (XRD) showed an ionic interaction between ALG-CS leading to their excellent compatibility. Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) results suggest increased ALG-CS scaffold's thermal stability. In-vitro biodegradation behavior demonstrated controlled degradation with lysozyme. The swelling ratio was highest in the first hour and decreased slowly with time, and the porosity analysis showed a high degree of porosity. The ALG-CS scaffold showed sustained drug availability and minimized re-application, which contributes to effective healing and treatment. The blood compatibility and whole blood clotting ability of the scaffold significantly improved after incorporating the drug. Calcein AM, Propidium iodide, was used for live and dead cell staining, which confirmed that fabricated ALG-CS-CHY scaffolds are biocompatible and facilitate cell growth and cell proliferation. In-vivo and in-vitro observations show that the experimental group treated using the ALG-CS-CHY reduces the period of re-epithelization, accelerated fibroblast cell migration, and contracted wound significantly (p < 0.001) compared to other groups. ALG-CS-CHY scaffolds also increased collagen deposition, hexosamine synthesis, accelerates angiogenesis, and recruiting immune cells at the site of a wound. These results suggest ALG-CS-CHY scaffold serves as an effective dressing for dermal wound management.