Abstract
Poly(ε-caprolactone) (PCL) membranes have been widely used in guided tissue regeneration (GTR) and guided bone regeneration (GBR). In addition, hydroxyapatite is the major inorganic component and an essential composition of hard bone and teeth. Recently, numerous studies have demonstrated that strontium-substituted hydroxyapatite (SrHA) not only enhances osteogenesis but also inhibits adipogenesis of mesenchymal stem cells. Therefore, SrHA incorporated into PCL could be an alternative material for GBR. In this study, strontium-substituted hydroxyapatite nanofibers (SrHANFs) were fabricated by a sol–gel route followed by electrospinning. We then fabricated PCL–SrHANF membranes as cell culture substrates and assessed the cellular behavior of osteoblast-like cells. Based on the observations of alkaline phosphatase (ALP) activity, bone sialoprotein (BSP) and osteocalcin (OCN) immunofluorescence staining, and Alizarin Red-S staining of cells cultured on the PCL–SrHANF and PCL membranes, we concluded that SrHANFs can promote the differentiation and mineralization of osteoblast-like cells and that PCL–SrHANF membranes have potential for GBR applications.
Highlights
Membranes are mainly used for guided tissue regeneration (GTR) or guided bone regeneration (GBR) in the treatment of periodontitis
MG63 osteoblast-like cells on membranes. These results indicate that the presence of strontium-substituted hydroxyapatite nanofibers (SrHANFs) in the PCL membranes promotes osteoblast differentiation and activity
An organic–inorganic composite membrane for guided bone regeneration was fabricated for the first time by incorporating fragments of strontium-substituted hydroxyapatite nanofiber into PCL
Summary
Membranes are mainly used for guided tissue regeneration (GTR) or guided bone regeneration (GBR) in the treatment of periodontitis. The high hydrophobicity of PCL relative to that of natural extracellular matrix (ECM) leads to poor cell behavior [3]. Lee et al showed that the incorporation of bioactive glass can significantly enhance cell activities on PCL membranes, indicating favorable osteoconductivity and osteoinductivity for GBR [7]. Shor et al found that PCL–HA scaffolds had significantly higher compressive modulus than did PCL scaffolds. They found that PCL–HA scaffolds had higher ALP activity and showed more mineralization of matrix than PCL scaffolds [8]
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