Abstract
Scaffolds providing a 3D environment which can effectively promote the adhesion, proliferation and differentiation of cells are crucial to tissue regeneration. In this study, the polyllactic acid (PLLA) scaffold with hierarchical pore structural was fabricated via two-step thermally induced phase separation (TIPS). To mimic both physical architecture and chemical composite of natural bone extracellular matrix (ECM), gelatin fibers were introduced into the pores of PLLA scaffolds and formed 3D network structure via TIPS. Human adipose tissue-derived stem cells (ADSCs) were harvested and seeded into PLLA/gel hybrid scaffolds and cultured in vitro for biocompatibility assay. The surface morphology, porosity and compressive modulus of scaffolds were characterized by scanning electron microscopy (SEM), density analysis and compression test respectively. The results showed that hybrid scaffolds had high porosity (91.62%), a good compressive modulus (2.79 ± 0.20 MPa), nanometer fibers (diameter around 186.39~354.30 nm) and different grades of pore size from 7.41 ± 2.64 nm to 387.94 ± 102.48 nm. The scaffolds with mild hydrolysis by NaOH were modified by 1-ethyl-3-(3-dimethyl ami-nopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS). Gelatin was performed onto PLLA scaffold via TIPS aiming at enhancement cell-material interaction. In comparison with PLLA scaffold, the PLLA/gel scaffold had better biological performance and the mechanical properties because the gelatin fibers homogeneously distributed in each pore of PLLA scaffold and formed 3D network structure.
Highlights
Bones are rigid and important organs that support and protect the various organs of the body, produce blood cells, store minerals and sever multiple other functions
Autogenous bones, allogenic/allogenous bones, and artificial bone substitutes are usually used as transplanted materials for bone defects, which have their respective strong and weak points [4,5]
The poly-L-lactic acid (PLLA) scaffold with hierarchical pore structural was fabricated via two-step thermally induced phase separation (TIPS). To mimic both the physical architecture and chemical composite of natural bone extracellular matrix (ECM), gelatin fibers were introduced into the pores of PLLA scaffolds and formed 3D network structure via TIPS
Summary
Bones are rigid and important organs that support and protect the various organs of the body, produce blood cells, store minerals and sever multiple other functions. Scaffolds and growth factors are three key materials of tissue engineering. How to obtain enough ADSCs efficiently and induce osteoblast differentiation stably is the principal issue of becoming ideal seed cells for bone tissue engineering. Fabrication of scaffold materials, as the simulation of extracellular matrix (ECM), is one of the important fields of bone tissue engineering. Gelatin is a partially denatured derivative of collagen [10,11,12,13], which has low immunogenicity, low cost and can be degraded entirely in vivo It has some inherent disadvantages, such as poor mechanical strength and hydrolysis. The biocompatibility of the scaffold and the cell morphology, viability and osteogenic differentiation were all investigated
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
