Fabrication and Application of Low-cost Microfluidic Analytical Devices Based on Paraffin-Heptane Treated Paper Substrate

Abstract

Paper is an ideal substrate material for low-cost diagnostic devices and shows a great potential in the fabrication of microfluidic paper-based analytical devices( μPADs). As one of the hot research fileds,a variety of materials and techniques have been proposed to fabricate μPADs. In this paper,we introduced a cheap,simpler and faster fabrication procedure,in which paraffin-heptane solution was used,toward this target. It is confirmed that as-developed protocol could be used for the simultaneous diagnostics of protein, glucose and pH. In addition,it is also applicable for quantitative analysis. The detection ranges of fabricated μPAD for bovine serum albumin and glucose were 2. 0 × 10- 5~ 1. 00 × 10- 4mol / L and 0. 02 ~ 0. 1 mol / L, respectively. This paper also preliminarily studied the spreading phenomena of microfluidic channel barrier during heating treatment. We found that the thickness of barrier followed a linear discipline.