Fabric phase sorptive extraction of monomers released from dental resins in alcoholic beverages prior to their HPLC-UV determination

Abstract

Fabric Phase Sorptive Extraction (FPSE) combined with High Pressure Liquid Chromatography coupled to UV (HPLC-UV) was applied for the simultaneous determination of organic monomers bisphenol-A (BPA), bisphenol A glycerolate dimethacrylate (Bis-GMA), triethylene glycol dimethacrylate (TEGDMA), and urethane dimethacrylate (UDMA)]that can be released from dental resins [in alcoholic beverages. The FPSE procedure was optimized with regards to the critical parameters that affect the performance of the method: a) FPSE membrane type and size, b) sample loading volume c) elution solvent, d) volume of elution solvent, e) stirring rate during extraction and elution, f) extraction time, and g) desorption/elution time. The developed FPSE-HPLC-UV method was subsequently validated in terms of linearity, trueness, precision and selectivity, showing satisfactory results. The limits of detection (LOD) and limits of quantification (LOQ) were equal to 0.06 ng/μL and 0.2 ng/μL, respectively. Relative recoveries ranged between 92.0% and 99.2% in white wine samples, and between 85.4% and 95.0% in vodka samples for within-day assay (n = 5). For between-day assay (n = 3 × 4), recoveries ranged between 92.0% and 101.8% in white wine samples, and between 88.4% and 95.2% in vodka samples. The method was successfully implemented in the investigation of monomers released from alcoholic beverages incubated with dental resins for a period of 24 h and 7 days.