F2-Isoprostane Levels in Deoxycorticosterone Acetate (DOCA)-Salt Induced Hypertensive Rats Administered with Coffee-Corn Mixture

Abstract

The purpose of this study is to analyze F2-Isoprostane levels in Deoxycorticosterone Acetate (DOCA)–Salt induced hypertensive rats that is given Coffee-Corn mixture. Measurement of blood pressure was carried out by non-invasive methods using CODA instruments. The coffee-corn mixture was made from Robusta (Coffea canephora) obtained from Kaliwining, Jember Regency, and Yellow Corn (Zea Mays) bought from the local market in Jember, East Java, Indonesia. F2-Isoprostane levels were obtained by ELISA examination. The mice’s blood pressure was measured using a non-invasive CODA® blood pressure gauge. Serum F2-isoprostane levels were measured using an F2-isoprostane-ELISA kit that was read with an Elisa reader at a wavelength of 450nm. The results showed that there was a decrease in mean systolic blood pressure in each treatment group, with the most decrease by 41.88mmHg occured in the group that was given a coffee-corn mixture with a ratio of 50% Robusta coffee (Coffea canephora) Kaliwining and 50% yellow corn (Zea Mays). The highest decrease in diastolic blood pressure by 35.25mmHg was also attained in the same group. From the results of serum F2 isoprostrane level examination, there was a decrease in the average level of serum F2 isoprostrane in the treatment group with the most reduction by 51.34pg/mL occured in the group given a coffee-corn mixture with a ratio of 50% Robusta (Coffea canephora) Kaliwining and 50% yellow maize (Zea Mays). Coffee-corn mixture can reduce blood pressure in hypertensive mice through anti-oxidant effects.