Abstract
To investigate the effect of the Quorum Sensing (QS)-I system on the expression of virulence factors in Shiga toxin producing and verotoxin-producing Escherichia coli (STEC and VTEC), the yenI gene from Yersinia enterocolitica was cloned into E. coli F18ab 107/86. Recombinant E. coli transformed with yenI produced acyl-homoserine lactone synthase (AHL), as measured using cross-streaking assays with the reporter biosensor strain Chromobacterium violaceum CV026. The AI-1 positive recombinant F18ab E. coli exhibited impaired expression of flagella, decreased motility, reduced biofilm formation and AI-2 production, as well as attenuated adherence and invasion on IPEC-J2 cells. This study provides new insights to the crucial function of AI-1 in regulating STEC virulence.
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