Abstract
1. 1. F 1 and OSCP, soluble coupling factors isolated from beef-heart mitochondria, form a complex. 2. 2. The binding between F 1 and OSCP is weakened by (NH 4) 2SO 4, but not enough to allow complete separation of F 1 and OSCP in a linear sucrose gradient. Complete separation is achieved when both (NH 4) 2SO 4 and deoxycholate are present. 3. 3. F 1· X, a coupling factor also isolated from beef-heart mitochondria, can be separated in F 1 and OSCP activities in the presence of (NH 4) 2SO 4 and deoxycholate by centrifugation in a linear sucrose gradient or by gel filtration on a Sephadex G-100 column. 4. 4. F 1· X preparations of low specific activity contain a contaminating protein. This protein can be dissociated from the complex by (NH 4) 2SO 4. F 1· X preparations with a high specific activity are virtually free of this contaminating protein. 5. 5. F 1· X preparations contain approximately 30% free F 1. 6. 6. Analytical gel electrophoresis of F 1· X, F 1 and OSCP in the presence of sodium dodecyl sulphate indicates that apart from F 1 and OSCP no other components are present in pure F 1· X.
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