F1 Hybrid Production using Doubled Haploid of Microspore-derived 'Moriguchi' Radish (Raphanus sativus L.)

Abstract

F1 hybrids were produced by using doubled haploid radishes, which were derived from microspore culture and maintained their homozygosity by selfing through bud-pollination three days before anthesis. The bud-pollination of doubled haploid was also undertaken to obtain seeds of F1 hybrids. The regenerants developed weak roots with small diameters and small leaves. However, good growth was restored in F1 hybrids that were crossed among the doubled haploid progenies of the regenerants. They grew uniformly and showed hybrid vigour. It was proven that haploid breeding was effective to establish a genetically fixed radish (Raphanus sativus L.) plant and a new type of 'Moriguchi' radish with a red, long, slender root.