Extremely-low frequency magnetic field exposure for simulating geomagnetic pulsations in Alexandrium pacificum and Gymnodinium catenatum cultures

Abstract

Growth and chain formation in cultures of the chain-forming dinoflagellates Alexandrium pacificum and Gymnodinium catenatum were previously found to be susceptible to space weather variables. A clock drive was used to deliver a frequency of 0.5 Hz and central amplitude of 7 µT in order to perform in vitro simulation of geomagnetic pulsations (composed of extremely low-frequency magnetic fields, ELFMF) which occur during high geomagnetic activity (GMA) periods. Short-term exposure (hours) to this ELFMF increased relative cell growth around 10 nT of naturally occurring GMA. Relative growth outside these intervals gradually approached 0% or was negative for G. catenatum. Differential survival to a subsequent shock was inversely related to growth, and minimal survival coincided with the same 10 nT interval. Relative growth and survival displayed opposite hormetic curves towards GMA: inverted U-shaped for growth, and J-shaped for survival. After exposure to this ELFMF, positive phototaxis response was not lost, but the percentage of cells swimming was slightly reduced. Long-term exposure (days) increased relative growth in A. pacificum but reduced in G. catenatum when low GMA was taking place. These alterations in growth were both associated with a reduction in the cellular pool of mycosporine-like amino acids (MAAs). MAAs that are more susceptible to oxidation were more reduced than those resistant, highlighting that an ELFMF can act by increasing cellular oxidative stress status. The higher susceptibility of G. catenatum found is in compliance with the previous association of its natural populations at the western Iberia coast with periods of solar activity minima and GMA minima.