Extrato das folhas de Arrabidaea chica (Fridericia chica) mostrou potencial ação no combate à cardiotoxicidade induzida pela doxorrubicina

M.S.O Barreto,D.A Gomes,N.C.R Corrêa,J.L Carvalho,A.M Goes,F.C Silva,A.F.R.M Michel,M.M Melo

doi:10.1590/1678-4162-12122

Abstract

Extrato das folhas de Arrabidaea chica (Fridericia chica) mostrou potencial ação no combate à cardiotoxicidade induzida pela doxorrubicina

Highlights

Both cell cultures were subjected to the following treatments, in three independent experiments performed in triplicates: control group; A. chica extract (AC) group; C. sinensis extract (CS) group; Dox group; AC-Dox group and CS-Dox group
The analysis showed one which was identified at 287.079 [M +h]+m/z corresponding to kaempferol
For propidium iodide (PI), 6x105 cardiomyocytes and 1x105 MDA-MB-231 cells were plated in 6-well plates

Summary

Introduction

Both cell cultures were subjected to the following treatments, in three independent experiments performed in triplicates: control group (cells incubated only with media, no plant extract, no dox); AC group (cells incubated with 0, 12.5, 25, 50, 100, and 200μg/ml of AC; without dox); CS group (cells incubated with 0, 12.5, 25, 50, 100, and 200μg/ml of CS; without dox); Dox group (no herbal extracts; 5μmol/l dox incubation for 2, 4, 16, and 24h); AC-Dox group (cells incubated with 0, 12.5, 25, 50, 100, and 200μg/ml of AC; 5.0μmol/l dox) and CS-Dox group (cells incubated with 0, 12.5, 25, 50, 100, and 200μg/ml of CS; 5.0μmol/l dox). Ventricular cardiomyocyte isolation from neonatal Wistar rats was performed as described earlier (Ott et al, 2008) and human mammary adenocarcinoma cell line MDA-MB-231 was used. For PI, 6x105 cardiomyocytes and 1x105 MDA-MB-231 cells were plated in 6-well plates. After 24h of seeding, fresh media was added to each cell type containing the mentioned concentrations of herbal extract.

Results

Conclusion