Abstract
Extraordinary transmission based axial imaging (EOT‐AIM) for cell microscopy is reported. EOT‐AIM uses linear arrays of nanoapertures, each of which samples target fluorescence up to a preset axial distance from surface, in combination with wide‐field microscopy for acquisition of lateral images. Current design of nanoapertures provides EOT‐AIM with axial super‐resolution that is as small as 20 nm for a depth range of 500 nm. Experiments were performed for the measurement of the axial distribution of ganglioside in mouse macrophage (RAW264.7) cells using FITC‐conjugated cholera toxin subunit B. The results were successfully confirmed with conventional confocal and total internal reflection fluorescence microscopy.
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