Abstract
This research aims to analyze the effect of the extract from a native plant from Caatinga, Allamanda blanchetti, in the induction of resistance mechanisms in a sugarcane variety smut-susceptible. Initially, it carried out a phytochemical analysis to know the main plant compounds used in this study. Diverse chemicals content in ethanolic extract from A. blanchetii were detected by thin-layer chromatography (TLC). Flavonoids, were more abundant compounds following by terpenes, stereroids and saponins. Under greenhouse conditions the sugarcane plants, SP-791011 (smut-susceptible), were sprayed with extracts from A. blanchetti extracted at cold at concentrations of 1000 ppm and acilbenzolar-S-metil (ASM) (100 mg/L). Leaves were collected at 0, 24 and 48 hours after spraying and used in the RT-PCR analysis for to identify the defense gene expression. Change in gene expression were observed in the different treatments, especially in the expression of pathogenesis-related (PR) genes. The extract of A. blanchetti induced an increase in the glucanase expression and was more effective than ASM inducer. SNPR1 gene show increased in the two treatment. The results indicate that A. blanchetti extracts was able to activate the resistance mechanism as observed in resistant plants. This paper is the first report about the use of Caatinga natural plant extracts inducing resistance genes against Sporisorium scitamineum in sugarcane susceptible genotype.
Highlights
Sugarcane (Saccharum spp.) is a major crop in Brazil and in many countries
The experiment was conducted in a greenhouse at the Instituto Agronômico de Pernambuco (IPA) and Biochemistry Laboratory of the Universidade Federal de Pernambuco (UFPE), Recife, Brazil
Flavonoids were more abundant compounds following by terpenes, steroids and saponins (Table 2). This results showed that plant extract contain molecules with potential for the application against smut disease
Summary
Sugarcane (Saccharum spp.) is a major crop in Brazil and in many countries. It is one of the most important crop for the sugar production and renewable biofuel energy worldwide. The sugarcane production has been affected by many biotic and abiotic stresses, among them fungal diseases are one of the most important. Induced resistance (IR) can be activated in plants by several substances, preventing or delaying pathogen entry or its subsequent activity in plant tissues, by their own defense mechanisms. Several inductor agents can induce the production of “signals” in plant tissue, triggering reactions that will result in a long-term protection against a wide range of jas.ccsenet.org
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