Abstract
AbstractThis work explores the use of water‐in‐supercritical carbon dioxide (scCO2) microemulsions for the extraction of polar metabolites from plants. Stevia rebaudiana Bertoni leaves and polyethylene glycol trimethylnonyl ether (TMN) surfactants were selected for a case study. A CO2‐water‐TMN 10 mixture at 35 °C and 30.0 MPa extracted 7 mg target analyte/g dry leaves. The extraction was proven to occur due to a water‐surfactant liquid solution rather than a water‐in‐CO2 microemulsion. Using a modified extraction setup, the microemulsion was created prior to extraction. TMN 6 was able to dissolve enough water in CO2 to extract steviol glycosides, in detectable but very small amounts.
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