Extraction, Quantification, and Cytokine Inhibitory Response of Bakuchiol in Psoralea coryfolia Linn.

Deepak Khuranna,Showkat Rasool Mir,Ajaz Ahmad,Parvaiz Ahmad,Muneeb U Rehman,Mohd Mujeeb,Mohamed Soliman Elshikh,Mohd Aqil,Mona S Alwahibi,Sanchit Sharma

doi:10.3390/separations7030048

Deepak Khuranna, Showkat Rasool Mir + Show 8 more

Open Access

https://doi.org/10.3390/separations7030048

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Journal: Separations	Publication Date: Sep 11, 2020
Citations: 7	License type: CC BY 4.0

Affiliation: Jamia Hamdard, King Saud University

Abstract

(1) Background: The present investigation studies the optimization of extraction, quantification, and cytokine inhibitory effects bakuchiol (BKL) in Psoralea coryfolia Linn. (2) Methods: The seeds of Psoralea coryfolia cleaned, dried, and powdered. Different separation methods maceration, reflux, Soxhlet, and ultrasonic assisted extraction (UAE) were employed for the isolation of BKL by five pure solvents. The quantity of BKL was measured by high-performance liquid chromatography (HPLC) method to determine the highest yield percentage. The effect of optimized BKL was then tested in an animal model of sepsis induced by lipopolysaccharides (LPS). (3) Results: The UAE method was found to be the best among tested separation methods and yielded highest percentage of BKL in petroleum ether extract. Septic rats showed a significant elevation in levels of biochemical markers like AST, ALT, ALP, BIL, SCr, and BUN in plasma. Proinflammatory cytokines (TNF-α and IL-1) levels were also increased in LPS-induced animals. BKL has been found to significantly reverse these elevated levels as compared to the LPS-induced animals. (4) Conclusion: The present results suggest that BKL has positive effects when administered in animals with pathogenic shock by decreasing the circulating levels of biomarkers. Further studies are necessary to explore the clinical implications of such findings.

Highlights

The fabaceae plant, Psoralea corylifolia L., is a medicinal plant widely distributed in India, China, and Southeastern Asian countries [1,2]
Ultrasonication assisted extraction (UAE) technique was found out to be the best method for highest extraction of BKL (6.98%, w/w) with petroleum ether being the best solvent for its extraction
(UAE) was selected further for BKL extraction as it gives the highest yield of BKL and it requires less time being highly efficient as compared to the other extraction methods (RT 24.5 min)

Summary

Introduction

The fabaceae plant, Psoralea corylifolia L., is a medicinal plant widely distributed in India, China, and Southeastern Asian countries [1,2]. Production) [3,4] It grows throughout the plains of India, especially in the semiarid regions of Rajasthan and eastern districts of Punjab, adjoining Uttar Pradesh. It is found throughout India in Himalayas, Dehradun, and Karnataka [1,5]. This plant is widely distributed in the tropical and subtropical regions of the world, especially China and southern Africa [2].

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