Abstract
Crude polysaccharide was prepared from Crassostrea rivularis by 30% (w/v) potassium hydroxide solution at 90°C for 120 min. Three fractions (OG1, OG2, and OG3) were purified by DEAE‐52 cellulose and Sepharose 2B gel column chromatography. The chemical structures were determined using gas chromatography (GC), high‐performance gel permeation chromatography (HPGPC), Fourier‐transform infrared (FT‐IR) spectroscopy, and 1H and 13C nuclear magnetic resonance (NMR) spectroscopy. The results indicated that OG1 was composed of rhamnose and little mannose (8.71%), the ratio of Rha: Gal: Xyl: Fuc in OG3 were 14:5.5:3:1. And their average molecular weights (Mw) were about 1.66 × 106 and 2.33 × 106 Da, respectively. OG2 was composed only of glucose (98.23%), which means it was glycogen. OG2 was consisted mainly of →4)‐ α‐D‐Glc‐(1→, with the branch chain every 6.5 glucose residues on average, which is →4,6)‐α‐D‐Glc‐(1→ and trace amount of α‐D‐Glc‐(1→ branched units. The Mw was 2.27 × 106 Da. It provides the bases for the bioactivity research.
Highlights
Mollusks are an excellent source of high-quality nutrition in China as well as other parts of the world and have been used as food products and traditional Chinese medicinal drugs for centuries
Glycogen content was detected by anthrone-sulfuric acid colorimetry according to Chen, Yang, and Gu (2005), and polysaccharide content was measured by the phenol- sulfuric acid method using glucose as the standard (Dubois, Gilles, Hamilton, Rebers, & Smith, 1956)
The glycogen content in C. rivularis was 4.92 g/100 g wet weight, which is less than the glycogen content in C. gigas (7.05 g/100 g wet weight, Chen et al, 2005)
Summary
Mollusks are an excellent source of high-quality nutrition in China as well as other parts of the world and have been used as food products and traditional Chinese medicinal drugs for centuries. Crassostrea rivularis lives in the conjunction regions of rivers and the sea. It is the main economical mollusk along the South China Sea and often aquacultured in Guangdong, Guangxi and Hainan provinces. It is desired for its flavor, protein, low fat, and high glycogen content. In order to research the sobering-up and antifatigue functions of polysaccharide from C. rivularis, we isolated the polysaccharide and analyzed the chemical structure in this study. The preliminary characterizations were investigated using high-performance gel permeation chromatography (HPGPC), Fourier-transform infrared (FT-IR) spectroscopy, and 1H and 13C nuclear magnetic resonance (NMR) spectroscopy
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