Extraction, Purification and Characterization of Peroxidase from Okra (Abelmoschus esculentus)

Abstract

This study was aimed to extract peroxidase enzyme from the okra plant. The enzyme was extracted by using sodium phosphate buffer (pH 6.5, 0.2 M). The crude extract then precipitated at the saturation range between 20 – 70 % and dialyzed. Further purified carried out through ion exchange chromatography using DEAE-Cellulose column (2.5 × 40 cm). The purified peroxidase had a molecular weight of 43.651KDa, and optimum pH and temperature for enzyme activity about 6.5 and 55°C using pyrogallol and H2O2 as substrate. The specific activity, fold of purification and yield of purified peroxidase were 87.5U/mg, 6.57 and 33.30% respectively.