Extraction, phytochemical screening and antioxidant potential of extract of Eulophia herbacea

Abstract

A family of extremely reactive molecules called reactive oxygen species (ROS) are produced during the metabolism of oxygen. Superoxide radicals, hydroxyl radicals, and hydrogen peroxide molecules are among the ROS that are frequently produced as byproducts of biological processes or as a result of external stimuli. There are several there is evidence that ROS play a role in the emergence of degenerative illnesses. There is evidence that substances, particularly those derived from natural sources, can protect against free radicals. Traditional medicines with therapeutic utility have been used since antiquity and are still contributing a significant role in the primary health-care system. Thus this article focused on the extraction, phytochemical and detection of antioxidant potential of Eulophia herbacea plant extract. The phytochemical screening revealed that the plant is a rich source of different secondary metabolites like flavonoids, saponins, carbohydrates, proteins phenol, glycosides, diterpenes and tannins. The extract only has flavonoid content with value 0.385 mg/100 mg of dried extract. The total phenolic content is only estimated in hydroalcoholic extract of Eulophia herbacea which is found to be 0.674mg/100mg of dried extract. The anti-oxidant activity was performed using DPPH method. The percentage inhibition seen to be highest this is 79.63% at maximum concentration of 100µg/ml. Beyond that IC50 value calculations reveal that Eulophia herbacea extract have comparable antioxidant potential with IC50 value for ascorbic acid is 18.69 and for plant extract it is 107.42.