Abstract

HIGHLIGHTS Bromelain was extracted from crown, core, and peel waste of pineapple. Ammonium sulphate (NH4)2SO4 precipitation method was used as a simple and cost-effective approach for partial purification of bromelain. A purification of 4.34, 2.75 and 2.59-fold was achieved for crown, core and peel bromelain samples respectively. Enzyme activity recovery of 170.41 %, 192.37 % and 337.59 % was achieved for crown, core, and peel bromelain, respectively.

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