Abstract
Herein, we optimized eco-friendly extraction parameters to get the maximum yield of a novel polysaccharide-based mucilage (SSH) from seeds of Salvia spinosa. The dependency of the extraction yield of SSH on the pH of the extraction medium (pH 6-8), extraction temperature (25-75 °C), seed/water ratio (1:10-1:40 w/v), and seed–water contact time (1-4 h) was evaluated using response surface methodology–Box Behnken design (RSM–BBD). A second-order polynomial equation provided the best fit to the studied response with p < 0.0001. The optimum conditions to achieve the maximum yield of SSH (7.35%) were at pH 7, extraction temperature of 50 °C, seed/water ratio of 1:25 w/v, and seed–water contact time of 2.5 h. Scanning electron microscopic analysis of SSH revealed its superporous nature.
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