Abstract
The present study demonstrates the comparative study between conventional batch extraction and nonconventional three phase partitioning (TPP) for the first time to extract potent biomarker compound corosolic acid from the leaves of Lagerstroemia speciosa. The impact of various TPP process parameters such as time, solute to solvent ratio, ammonium sulphate concentration percentage, slurry to 2-Methylpropan-2-ol ratio and pH were investigated to enhance the yield of corosolic acid. The various optimized parameters were solute to solvent ratio 1:40 (w/v), ammonium sulphate concentration 40% (w/v), slurry to 2-Methylpropan-2-ol ratio 1:1 (v/v), time 120 min and pH 5. The highest extraction yield of corosolic acid obtained under optimized condition of TPP was (9.4 ± 0.4 mg/g). Considering the conventional technique(Soxhlet) as a reference method for comparison, TPP yielded 67.14% in 2 h, whereas batch resulted in 48.57% in 1h 30 min. Also, Lagerstroemia speciosa extracts obtained from various extraction technique showed a good amount of total phenolic content and antioxidant activity in equivalence with their respective standards. Hence, the proposed method was found to be more efficient, simple, and a green technique for maximum extraction of corosolic acid from Lagerstroemia speciosa.
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