Abstract
Optimization of steviol glycosides extraction from Stevia rebaudiana Bertoni leaf was carried out by investigating the effects of isopropyl alcohol concentration (60%, v/v), time (6-24min), temperature (30°C) and sonic power (300-480W) on extraction of rebaudioside A from Stevia rebaudiana leaves and decolorization of the extract by polymer (Separan AP30 and Resin ADS-7). The results showed that isopropyl alcohol was suitable for the extraction of rebaudioside A from Stevia rebaudiana leaves and the yield of rebaudioside A achieved 35.61g/100g when the output power was 360W and treatment time was 18min. The sonication had influence on the particle size of stevia leaf and the color of the extracted solution. As the sonication intensity increased, the particle size decreased. The colour of differently treated stevia solutions were significantly different (P < 0.05). Separan AP30 and adsorption resin ADS-7 were performed to remove the colour impurity. The results showed that more than 65% of the coloured impurity was removed by Separan AP30 combined with Calcium oxide (CaO).
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