EXTRACTION OF POLYSACCHARIDES FROM LINGZHI BY ULTRASONIC-ASSISTED ENZYMATIC METHOD

Abstract

The medicinal values of polysaccharides (PS) in Lingzhi have been shown to lie in many anti-cancer effects and good benefits for human health. Lingzhi, which is rich in healthy PS, has been used more and more commonly in Vietnam in recent years. In the present work, ultrasonic-assisted enzymatic extraction (UAEE) was used for extraction of PS from Lingzhi. The experiments were conducted according to a Box-Behnken design (BBD), with four independent variables: solvent temperature, ultrasonic power, pH, and ultrasonic irradiation time. The results showed that the best adequate extraction conditions were extraction time of 144 min, extraction temperature of 55°C, ultrasonic power of 240W, pH 7.9, and ultrasonic irradiation time of 144 min. Under these conditions, the predicted optimal yield was 3.716%. Whereas by following the optimized conditions, the experimental yield of PS was 3.65% ± 0.14, which was in good agreement with that of the prediction. Compared to the hot water extraction (HWE) method, ultrasonic-assisted extraction (UAE) method and enzyme-assisted extraction (EAE) method, the yield of PS obtained by UAEE was favorable. The PS yield obtained by HWE, and EAE were 1.96% and 3.10%, respectively. These results demonstrated that UAEE was an appropriate and effective extraction of polysaccharides from Lingzhi.