Abstract
Abstract Extraction with supercritical carbon dioxide (SC-CO2) which is known as a clean technology was carried out to extract oil from melon (Cucumis melo) seeds. SC-CO2 extraction technique does not contaminate extracts. SC-CO2 is not a toxic and a flammable solvent. Phytosterols, natural and bioactive compounds, which is known to provide protection against various chronic diseases were examined in the seed oil by using gas chromatography – mass spectrometry (GC-MS). Stigmasterol and β-sitosterol were detected in the melon seed oil. SC-CO2 extractions were performed in a range of 30-55°C, 150-240 bar, 7-15 g CO2/min, 0.4-1.7 mm (mean particle size of the seeds) and 1-4 h. The optimal quantities of extracted oil, β-sitosterol and stigmasterol were 36.8 g/100 g seed, 304 mg/ kg seed and 121 mg/ kg seed, respectively, at 33°C, 200 bar, 11 g CO2/min, 0.4 mm and 3 h.
Highlights
As a result of the analysis with GC-MS, β-sitosterol and stigmasterol were found (Figure 1) in melon seed oil by comparison of their retention times according to the standards which have been purchased. 350-650 g/mol of measured molecular mass range in GC-MS and separating phytosterols from other compounds by saponification provided easy selectable peaks
Β-sitosterol and stigmasterol which are principal phytosterols were detected in melon seed oil
Melon seed oil was obtained by the supercritical CO2 extraction which is a clean and an effective method
Summary
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