Extraction of Phenolics and Flavonoids from Four Hosta Species Using Reflux and Ultrasound-Assisted Methods with Antioxidant and α-Glucosidase Inhibitory Activities.

Junwei He,Liangfa Wu,Li Yang,Chunlong Li,Boyuan Zhao,Janet Mifsud

doi:10.1155/2020/6124153

Abstract

The total phenolic and flavonoid contents (TPC and TFC) from the genus Hosta with antioxidant and α-glucosidase inhibitory activities were reported for the first time. Sixteen extracts from the aboveground and underground parts of the four Hosta species, including H. plantaginea, H. ventricosa, H. ensata, and H. albofarinosa, using reflux extraction (RE) and ultrasound-assisted extraction (UAE) techniques have high TPC and TFC with good antioxidant and α-glucosidase inhibitory activities. Furthermore, no significant differences on extraction yields, TPC, and TFC were found between RE and UAE techniques. Additionally, extracts from the aboveground parts of the four Hosta species had higher TPC, TFC, antioxidant, and α-glucosidase inhibitory activities compared to the underground parts by means of RE or UAE techniques. Lastly, the extracts of H. albo-marginata displayed a very remarkable α-glucosidase inhibitory activity compared to the positive control acarbose. The relationships of sixteen extracts of the four Hosta species were analyzed by RE and UAE techniques between extraction yields, TPC, TFC, antioxidant activity, and α-glucosidase inhibitory activity. The present study demonstrated that H. plantaginea, H. ventricosa, H. ensata, and H. albofarinosa could be new sources of natural antioxidants and antidiabetes for pharmaceutical and industrial purposes.

Highlights

Hosta Tratt. is a genus belonging to the family Liliaceae, which comprises about 50 species distributed mainly in the temperate and subtropical zones of East Asia and Russia, but most species have been found in China, Japan, and Korea, and most of them are commonly used for ornamentally purpose or traditional folk medicines [1, 2]
Extracts from the underground parts of four Hosta species had higher extraction yields compared to the aboveground parts (P < 0:01) by reflux extraction (RE) or ultrasoundassisted extraction (UAE) techniques
This work has shown that extracts from four Hosta species, including H. plantaginea, H. ventricosa, H. ensata, and H. albofarinosa, have high yields of TPC and TFC, as well as good antioxidant and α-glucosidase inhibitory activities

Summary

Introduction

Hosta Tratt. is a genus belonging to the family Liliaceae, which comprises about 50 species distributed mainly in the temperate and subtropical zones of East Asia and Russia, but most species have been found in China, Japan, and Korea, and most of them are commonly used for ornamentally purpose or traditional folk medicines [1, 2]. Is a genus belonging to the family Liliaceae, which comprises about 50 species distributed mainly in the temperate and subtropical zones of East Asia and Russia, but most species have been found in China, Japan, and Korea, and most of them are commonly used for ornamentally purpose or traditional folk medicines [1, 2]. H. albofarinosa D.Q.Wang, which are widely cultivated in parks and/or commonly used as traditional Chinese medicines [1]. H. albo-marginata (Hook.) Ohwi originated in Japan and is cultivated as an ornamental plant in many regions of China [1]. The genus Hosta is a rich source of polyphenolics and flavonoids, which have been linked to antioxidant and antiinflammatory effects [2,3,4,5,6]. The total phenolic and flavonoid contents (TPC and TFC), as well as the antioxidant and α-glucosidase inhibitory activities, have not been reported from the genus Hosta, except that some flavonoids were moderate against the DPPH free radical scavenging activity in our previous articles [3,4,5,6]

Methods

Results

Conclusion