Abstract
The aim of the present study was to determine the phenolic profile, antioxidant capacities, inhibition of enzymes related to dermatological and neurodegenerative diseases, and cytotoxic properties of Acinos rotundifolius Pers. For this purpose, three different extraction methods were applied: Soxhlet extraction, ultrasonic-assisted extraction (UAE), and infusion (INF). The use of different solvents (ethyl acetate, methanol, water) has also been applied. The RP-HPLC-DAD data highlighted that Soxhlet extraction in MeOH/water is more effective at extracting flavonoids and phenolic acids. The UAE extract, which was richer in chlorogenic acid, exhibited a better scavenging capacity versus HO•, NO• and ABTS•+ were determined by UAE, whereas the one obtained via Soxhlet extraction in water had a better efficacy to eliminate O2•. The greatest inhibitory effects on AChE and tyrosinase enzymes were demonstrated by the infusion. The different composition of the extracts obtained with the different methods also influences the cytotoxic properties which were investigated by MTT test on HepG2 and OE-33 cell lines.
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