Extraction of peroxidase enzyme from different vegetables for biodetoxification of vat dyes

Abstract

Vat dyes are main sources for polluting the wastewater of textile and other industrial effluents. For removal of these dyes specific strategies and techniques are important. An enzyme known as peroxides has the capacity to absorb these dyes from an aqueous solution and also to decolorize the textile effluents. The current study aimed, biodetoxification and decolorization of vat dyes, in which three vat dyes, vat dye green 9, vat dye yellow 2, vat dye black 27, were investigated using peroxides enzymes from different vegetables sources at different condition. Decolorization efficiency of dyes was studied with the addition of ammonium sulphate and hydrogen per oxide in aqueous solution. The decolorization effect of peroxides was observed at different stages at different conditions, like at the beginning, before the incubation and after the incubation in hot water bath and observed on different wavelength using UV-VIS6000 spectrophotometer. Best decolorization efficiency was observed by Vat dye Black 27, i.e., 0.750 nm at 600 nm wavelength then Vat dye Green 9 showed least efficiency which has been increased by 50% and the most decrease in decolorization observed in vat dye green 9 after incubation 0.031 nm at 480 wave length. Current study suggests that the peroxides from the vegetables sources have the ability of decolorization and biodetoxification of vat dyes from textile dye effluent and other industrial wastewater and therefore, this enzymatic approach can be used for the removal and biodetoxification of vat dyes from the wastewater as this method is environmental friendly and can be performed on low cost and in short time.