Abstract
This study describes the comparison of three DNA extraction protocols for successful extraction of PCR amplifiable quality DNA from bones, antlers and feces samples of Sambar deer (Rusa unicolor). Three different DNA extraction protocols were compared in this study including Phenol–Chloroform (PC), column based Qiagen kit, and Guanidine hydrochloride (Gu-HCl) based in-house method. The effectiveness of the protocols was compared for higher success rate of PCR amplification from the extracted DNA. This study highlights that silica based indigenous DNA extraction protocol using Gu-HCl chaotropic salts yields better quality DNA with higher PCR amplification success rate.
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