Abstract
For the study of organic acids by gas chromatography the acids must first be extracted from the aqueous solution or protein-free physiological fluid as a complete group before chromatographic separation. This may be compared to liquid chromatography and paper and thin-layer chromatography where generally the aqueous fluid is studied directly without prior extraction. However, direct analysis of the fluid means that often it is not possible to be sure that a particular component is really an organic acid and not a neutral or basic component of the fluid and even with these techniques it would be preferable to undertake an initial extraction of the organic acids as a group before chromatography. This chapter is concerned with this extraction process and with the preparation of protein-containing fluids for extraction. In addition the methods are discussed for the concentration of the extracted acids prior to derivative formation and gas chromatography or separation by other chromatographic systems.
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