Abstract
Sugarcane trash (SCT) is an environmental concern in Indonesia. The chemical compositions of SCT can be used as benign materials, such as lignin-based biosurfactants, for improving enzymatic hydrolysis performance through the biorefinery concept, yet no one has worked on this. Therefore, the crux of this study is to evaluate lignin and lignin-based biosurfactant characteristics from two different acid-alkali extraction methods: microwave (SCT-A) and autoclave (SCT-B). Both extraction methods successfully isolated lignin with a total lignin content of 82 % and 25 % for SCT-A and 73 % and 35 % for SCT-B, respectively. Lignin was successfully processed into biosurfactant by grafting method, which was proved by ester groups in biosurfactants at 1700 cm−1 from FTIR and δC 163.9–164.4 ppm from 13C NMR. Lignin-based SCT-A can reduce water surface tension up to 50.2 mN/m from 58.8 mN/m (pure water), while the lignin-based SCT-B was 54.4 mN/m. Further studies are required to evaluate the effectiveness of lignin-based biosurfactants in enzymatic hydrolysis.
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