Abstract
Abstract An additional extraction/flotation method for the determination of light filth in whole wheat flour was validated through a collaborative study. A 50 g test portion is boiled in a 3% HCI solution. The mixture is washed with hot tap water on a No. 230 sieve. Then the residue is boiled in iso-propanol, transferred to a No. 230 sieve, and washed again. The residue is transferred to a Wildman trap flask using 40% isopropanol. The filth is isolated by flotation in mineral oil and a mixture of Tween 80 and Na4EDTA in 40% isopropanol. Average recoveries by 8 collaborators were 88.8 and 91.7% for insect fragments and rat hairs, respectively. The extraction/flotation method for determination of light filth in whole wheat flour has been adopted first action by AOAC INTERNATIONAL as an additional procedure to the AOAC Official Method 941.16, Filth in Grain Products.
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