Abstract
Hyaluronic acid have a high moisture preservation and biocompatibility characteristic, thus allowing various usage of this substance in pharmaceutical, medicinal, and skin care products. Present manufacturing process of hyaluronic acid requires extraction of animal cells or through other methods utilizing bacteria. The aim of this research is to investigate an alternative source of hyaluronic acid extraction using plant based which is aloe vera (A. Barbadensis). Three main parts of aloe vera (rind, mesophyll and gel) underwent several steps of extraction process and the result was compared to the sample of hyaluronic acid from goat brain. The evaluation including comparison of total carbohydrates, reducing sugars and degradation using heat treatment. The results of extraction were analyzed using UV-Spectrophotometer at 230 nm and compare to the extraction result of goat brain to ensure the presence of hyaluronic acid. It was found out that the rind part of aloe vera have the highest potential of high content of hyaluronic acid.
Highlights
Hyaluronic acid (HA) is a biological occurring polymer which has substantial biological functions in almost every organism (Necas, et al, 2008)
Hyaluronic acid occurred in a high molecular weight due to the repetition of glucuronic acid and Nacetylglucosamine that able to goes up to a thousand repetition even further as can be seen from figure 1
Anthrone method showed that all parts of aloe vera containing carbohydrate with rind has the highest absorbance, just below the absorbance of goat brain
Summary
Hyaluronic acid (HA) is a biological occurring polymer which has substantial biological functions in almost every organism (Necas, et al, 2008). HA can be found in skin, vitreous of the eye, umbilical cord, and synovial fluid, but it is present in body’s tissues such as skeletal tissues, heart valves, lungs, brain, and many others (Meyer K., Palmer, J.W., 1934). Resources to gain hyaluronic acid were commonly taken from various animal tissues such as human umbilical cords, rooster combs, bovine vitreous humor, and bovine synovial fluid Due to the grinding procedure and several repetition of using acid and organic solvents, both practical and mechanical issues will always happened in animal extraction in terms of cost and safety (Widner, et al, 2005)
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