Abstract
DNA extraction has been suggested as a main step affecting molecular DNA technology such as PCR and PCR-based methods. Therefore, researchers have used several modified protocols for efficient DNA extraction from bacteria. Results showed that high quantity and quality of isolated DNA from negative and positive bacteria (Escherichia. coli, Klebsiella. pneumonia, Pseudomonas. aeruginosa, Staphylococcus. aureus and Staphylococcus. epidermidis) by blood extraction kit to do hundreds of PCR-based reactions and also to be utilized in other DNA manipulation assay such as restriction digestion, drug-DNA interaction and methylation detection survey. This method is applicable to various species of bacteria isolated from diverse sources. The DNA yields were high and pure enough to be readily amplified by PCR and the PCR products were suitable for sequencing. In conclusion, we reduce the economic cost of extraction bacterial DNA to 50%.
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