Abstract
Abelmoschus manihot (L.) Medic has been used for many years in Chinese traditional medicine. In this study, supercritical CO2 plus a modifier was utilized to extract flavonoids from the flowers of Abelmoschus manihot (L.) Medic. The effects of temperature (40 °C–60 °C), pressure (10–30 MPa) and different concentrations of ethanol as modifier (60%–90%, ethanol:water, v/v) on major flavonol content and the antioxidant activity of the extracts were studied by response surface methodology (RSM) using a Box-Behnken design. The flavonol content was calculated as the sum of the concentrations of seven major flavonoids, namely rutin, hyperin, isoquercetin, hibifolin, myricetin, quercetin-3′-O-glucoside and quercetin, which were simultaneously determined by a HPLC method. The antioxidant activity was evaluated by a 2,2-diphenyl-1-picrylhydarzyl (DPPH) free radical-scavenging assay. The results showed that three factors and their interactions could be well fitted to second-order polynomial models (p < 0.05). At the optimal extraction conditions for flavonol content (20 MPa, 52 °C, and 85% ethanol content), the yield of flavonoids was 41.96 mg/g and the IC50 value was 0.288 mg/mL, respectively, suggesting the extract has high antioxidant activity. Furthermore, the anti-adipogenic activity of the extract on the 3T3-L1 cell line was investigated. The results indicated that it can downregulate PPARγ and C/EBPα expression at mRNA. In summary, in this study, we have established a cost-effective method for the extraction of flavonoids from the flowers of Abelmoschus manihot (L.) Medic using supercritical fluid extraction and the extracts exhibited potent antioxidant and anti-adipogenic effects, suggesting a possible therapeutic approach for the prevention and treatment of obesity.
Highlights
Abelmoschus manihot (L.) Medic is widely used in China
We investigated: (i) the effects of supercritical fluid extraction (SFE) extraction parameters including pressure, adipocyte differentiation which can in upregulate expression of enzymes and functional proteins temperature and ethanol[17], concentration the yieldthe of major flavonol and antioxidant activity using which are related to lipid accumulation and lipid metabolism
More and more people suffer from obesity, and research into natural anti-obesity products has become a hot topic in recent years [21]
Summary
Abelmoschus manihot (L.) Medic is widely used in China. The flowers are an important herbal medicine for the treatment of chronic renal disease [1,2], diabetic nephropathy [3], oral ulcers [4]. Adipocyte differentiation is a highly hypertension, coronary heart disease, cancer [14].factors, Obesity is characterized excessive regulated process, in which many kindsand of transcription signaling pathways by andanmiRNA are accumulation of fat cells in the human body [15]. It can be regulated by the differentiation of adipocytes required [15]. We investigated: (i) the effects of SFE extraction parameters including pressure, adipocyte differentiation which can in upregulate expression of enzymes and functional proteins temperature and ethanol[17], concentration the yieldthe of major flavonol and antioxidant activity using which are related to lipid accumulation and lipid metabolism. 2016, 21, 810and C/EBPα expression at mRNA using the 3T3-L1 cell line
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